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Nat Methods. 2016 Apr;13(4):319-21. doi: 10.1038/nmeth.3775. Epub 2016 Feb 29.

SuReSim: simulating localization microscopy experiments from ground truth models.

Author information

1
Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.
2
Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.

Abstract

Super-resolution fluorescence microscopy has become a widely used tool in many areas of research. However, designing and validating super-resolution experiments to address a research question in a technically feasible and scientifically rigorous manner remains a fundamental challenge. We developed SuReSim, a software tool that simulates localization data of arbitrary three-dimensional structures represented by ground truth models, allowing users to systematically explore how changing experimental parameters can affect potential imaging outcomes.

PMID:
26928761
DOI:
10.1038/nmeth.3775
[Indexed for MEDLINE]

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