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Redox Biol. 2016 Aug;8:298-304. doi: 10.1016/j.redox.2016.02.004. Epub 2016 Feb 13.

Direct spectrophotometric measurement of supra-physiological levels of ascorbate in plasma.

Author information

1
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: jordan.witmer@gmail.com.
2
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: wethereb@uni.edu.
3
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: brett-wagner@uiowa.edu.
4
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: juan-du@uiowa.edu.
5
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: joseph-cullen@uiowa.edu.
6
Department of Radiation Oncology, Free Radical and Radiation Biology Program, B180 Medical Laboratories, The University of Iowa, Iowa City, IA 52242-1181, United States. Electronic address: garry-buettner@uiowa.edu.

Abstract

BACKGROUND:

Supra-physiological concentrations of ascorbate, vitamin C, in blood, greater than 1mM, achieved through intravenous administration (IV), are being tested in clinical trials to treat human disease, e.g. cancer. These trials need information on the high levels of ascorbate achieved in blood upon IV administration of pharmacological ascorbate so appropriate clinical decisions can be made.

METHODS:

Here we demonstrate that in the complex matrix of human blood plasma supra-physiological levels of ascorbate can be quantified by direct UV spectroscopy with use of a microvolume UV-vis spectrophotometer.

RESULTS:

Direct quantitation of ascorbate in plasma in the range of 2.9mM, lower limit of detection, up to at least 35mM can be achieved without any sample processing, other than centrifugation.

CONCLUSIONS:

This approach is rapid, economical, and can be used to quantify supraphysiological blood levels of ascorbate associated with the use of IV administration of pharmacological ascorbate to treat disease.

KEYWORDS:

Ascorbate; Blood plasma; Spectrophotometric determination

PMID:
26928133
PMCID:
PMC4776628
DOI:
10.1016/j.redox.2016.02.004
[Indexed for MEDLINE]
Free PMC Article

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