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Am J Respir Crit Care Med. 2016 Aug 15;194(4):464-75. doi: 10.1164/rccm.201508-1678OC.

Critical Genomic Networks and Vasoreactive Variants in Idiopathic Pulmonary Arterial Hypertension.

Author information

1
1 Division of Allergy, Pulmonary, and Critical Care Medicine.
2
2 Department of Biomedical Informatics.
3
3 Division of Cardiology, University of Chicago, Chicago, Illinois; and.
4
4 Department of Medicine, Queen's University, Kingston, Ontario, Canada.
5
5 Department of Pediatric Medical Genetics, and.
6
6 Division of Allergy, Immunology, and Pulmonary Medicine, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee.

Abstract

RATIONALE:

Idiopathic pulmonary arterial hypertension (IPAH) is usually without an identified genetic cause, despite clinical and molecular similarity to bone morphogenetic protein receptor type 2 mutation-associated heritable pulmonary arterial hypertension (PAH). There is phenotypic heterogeneity in IPAH, with a minority of patients showing long-term improvement with calcium channel-blocker therapy.

OBJECTIVES:

We sought to identify gene variants (GVs) underlying IPAH and determine whether GVs differ in vasodilator-responsive IPAH (VR-PAH) versus vasodilator-nonresponsive IPAH (VN-PAH).

METHODS:

We performed whole-exome sequencing (WES) on 36 patients with IPAH: 17 with VR-PAH and 19 with VN-PAH. Wnt pathway differences were explored in human lung fibroblasts.

MEASUREMENTS AND MAIN RESULTS:

We identified 1,369 genes with 1,580 variants unique to IPAH. We used a gene ontology approach to analyze variants and identified overrepresentation of several pathways, including cytoskeletal function and ion binding. By mapping WES data to prior genome-wide association study data, Wnt pathway genes were highlighted. Using the connectivity map to define genetic differences between VR-PAH and VN-PAH, we found enrichment in vascular smooth muscle cell contraction pathways and greater genetic variation in VR-PAH versus VN-PAH. Using human lung fibroblasts, we found increased stimulated Wnt activity in IPAH versus controls.

CONCLUSIONS:

A pathway-based analysis of WES data in IPAH demonstrated multiple rare GVs that converge on key biological pathways, such as cytoskeletal function and Wnt signaling pathway. Vascular smooth muscle contraction-related genes were enriched in VR-PAH, suggesting a potentially different genetic predisposition for VR-PAH. This pathway-based approach may be applied to next-generation sequencing data in other diseases to uncover the contribution of unexpected or multiple GVs to a phenotype.

KEYWORDS:

pulmonary arterial hypertension; vasodilator responsive; whole-exome sequencing

PMID:
26926454
PMCID:
PMC5003329
DOI:
10.1164/rccm.201508-1678OC
[Indexed for MEDLINE]
Free PMC Article

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