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Front Pharmacol. 2016 Feb 18;7:27. doi: 10.3389/fphar.2016.00027. eCollection 2016.

NanoLuc Luciferase - A Multifunctional Tool for High Throughput Antibody Screening.

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Molecular and Cellular Biology Unit, Institut de Recherche Pierre Fabre, Centre d'Immunologie Pierre Fabre Saint-Julien-en-Genevois, France.
Biochemistry Department, Institut de Recherche Pierre Fabre, Centre d'Immunologie Pierre Fabre Saint-Julien-en Genevois, France.


Based on the recent development of NanoLuc luciferase (Nluc), a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic antibodies and antibody fragments could be characterized and quantified without purification. This system is very versatile allowing cellular and solid phase ELISA but also homogeneous BRET based screening assays, relative affinity determinations with competition ELISA and direct Western blotting. The new Nluc protein fusion represents a "swiss army knife solution" for today and future high throughput antibody drug screenings.


NanoLuc®; Western blot; affinity determination; bioluminescence resonance energy transfer (BRET); expression analysis; homogeneous assay; library screening; luciferase; phage display

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