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Virus Genes. 2016 Jun;52(3):309-16. doi: 10.1007/s11262-015-1273-y. Epub 2016 Feb 29.

Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells.

Author information

1
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic. cerny@paru.cas.cz.
2
Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 31, 370 05, České Budějovice, Czech Republic. cerny@paru.cas.cz.
3
Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic. cerny@paru.cas.cz.
4
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic.
5
Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 31, 370 05, České Budějovice, Czech Republic.
6
Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic.
7
The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK.

Abstract

A short upstream open reading frame (uORF) was recently identified in the 5' untranslated region of some tick-borne encephalitis virus (TBEV) strains. However, it is not known if the peptide encoded by TBEV uORF (TuORF) is expressed in infected cells. Here we show that TuORF forms three phylogenetically separated clades which are typical of European, Siberian, and Far-Eastern TBEV subtypes. Analysis of selection pressure acting on the TuORF area showed that it is under positive selection pressure. Theoretically, TuORF may code for a short hydrophobic peptide embedded in a biological membrane. However, expression of TuORF was detectable neither by immunoblotting in tick and mammalian cell lines infected with TBEV nor by immunofluorescence in TBEV-infected mammalian cell lines. These results support the idea that TuORF is not expressed in TBEV-infected cell or expressed in undetectably low concentrations. Therefore we can assume that TuORF has either minor or no biological role in the TBEV life cycle.

KEYWORDS:

Immunoblotting; Immunofluorescence; TBEV; TuORF; uORF

PMID:
26924586
DOI:
10.1007/s11262-015-1273-y
[Indexed for MEDLINE]

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