Format

Send to

Choose Destination
Plant Physiol Biochem. 2017 Jan;110:138-146. doi: 10.1016/j.plaphy.2016.02.010. Epub 2016 Feb 10.

1H NMR and GC-MS based metabolomics reveal nano-Cu altered cucumber (Cucumis sativus) fruit nutritional supply.

Author information

1
Bren School of Environmental Science & Management, University of California, Santa Barbara, CA 93106-5131, United States; University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, CA United States.
2
Materials Research Laboratory, University of California, Santa Barbara, CA 93106-5121, United States.
3
College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.
4
Bren School of Environmental Science & Management, University of California, Santa Barbara, CA 93106-5131, United States; University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, CA United States. Electronic address: keller@bren.ucsb.edu.

Abstract

It is imperative to study the interaction of nanoparticles residuals with crop plants in agricultural soils, due to the increased application of nanotechnology in agriculture. So far, a few studies have focused on the impact of nanoparticles on fruit quality and nutritional supply. In this work, a thorough and comprehensive analysis of metabolite changes of cucumber fruits from plants under nano-Cu stress was possible through the use of both 1H NMR and GC-MS. The results of supervised partial least-squares discriminant analysis from both platforms showed that cucumber fruit extracts samples were clearly grouped based on the nano-Cu level in soil. This indicates that the fruit metabolite profile was influenced by exposure to nano-Cu. GC-MS data showed concentrations of some sugars, organic acids, amino acids, and fatty acids were increased or decreased by nano-Cu. Several metabolites, such as methylnicotinamide (MNA), trigonelline, imidazole, quinolinate were only detected and quantified by 1H NMR. Our results showed that combining the two platforms provided a comprehensive understanding about the metabolites (nutrient supply) changes in cucumber fruits impacted by exposure to nano-Cu.

KEYWORDS:

Cucumber; GC–MS; Metabolomics; NMR; Nano-Cu

PMID:
26922143
DOI:
10.1016/j.plaphy.2016.02.010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center