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Nucleic Acids Res. 2016 May 5;44(8):3865-77. doi: 10.1093/nar/gkw116. Epub 2016 Feb 25.

Distribution of miRNA expression across human tissues.

Author information

1
Institute of Human Genetics, Saarland University, Medical School, Homburg, Germany.
2
Institute of Anatomy and Cell Biology, Saarland University, Medical School, Homburg, Germany.
3
Chair for Clinical Bioinformatics, Saarland University, Saarbruecken, Germany.
4
Department I of Internal Medicine and Center of Integrated Oncology, University Hospital of Cologne, Cologne, Germany Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Cologne, Germany.
5
Department of Internal Medicine III, University Hospital Heidelberg, 69120 Heidelberg, Germany German Center for Cardiovascular Research (DZHK), 69120 Heidelberg, Germany Klaus Tschira Institute for Integrative Computational Cardiology, D-69118 Heidelberg, Germany.
6
Siemens Healthcare, Hartmannstrasse 16, 91052 Erlangen, Germany.
7
Chair for Clinical Bioinformatics, Saarland University, Saarbruecken, Germany andreas.keller@ccb.uni-saarland.de.

Abstract

We present a human miRNA tissue atlas by determining the abundance of 1997 miRNAs in 61 tissue biopsies of different organs from two individuals collected post-mortem. One thousand three hundred sixty-four miRNAs were discovered in at least one tissue, 143 were present in each tissue. To define the distribution of miRNAs, we utilized a tissue specificity index (TSI). The majority of miRNAs (82.9%) fell in a middle TSI range i.e. were neither specific for single tissues (TSI > 0.85) nor housekeeping miRNAs (TSI < 0.5). Nonetheless, we observed many different miRNAs and miRNA families that were predominantly expressed in certain tissues. Clustering of miRNA abundances revealed that tissues like several areas of the brain clustered together. Considering -3p and -5p mature forms we observed miR-150 with different tissue specificity. Analysis of additional lung and prostate biopsies indicated that inter-organism variability was significantly lower than inter-organ variability. Tissue-specific differences between the miRNA patterns appeared not to be significantly altered by storage as shown for heart and lung tissue. MiRNAs TSI values of human tissues were significantly (P = 10(-8)) correlated with those of rats; miRNAs that were highly abundant in certain human tissues were likewise abundant in according rat tissues. We implemented a web-based repository enabling scientists to access and browse the data (https://ccb-web.cs.uni-saarland.de/tissueatlas).

PMID:
26921406
PMCID:
PMC4856985
DOI:
10.1093/nar/gkw116
[Indexed for MEDLINE]
Free PMC Article

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