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G3 (Bethesda). 2016 May 3;6(5):1191-200. doi: 10.1534/g3.116.028175.

Comparative and Transcriptome Analyses Uncover Key Aspects of Coding- and Long Noncoding RNAs in Flatworm Mitochondrial Genomes.

Author information

1
Howard Hughes Medical Institute Stowers Institute for Medical Research, Kansas City, Missouri 64110.
2
College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.
3
Stowers Institute for Medical Research, Kansas City, Missouri 64110.
4
Howard Hughes Medical Institute Stowers Institute for Medical Research, Kansas City, Missouri 64110 asa@stowers.org.

Abstract

Exploiting the conservation of various features of mitochondrial genomes has been instrumental in resolving phylogenetic relationships. Despite extensive sequence evidence, it has not previously been possible to conclusively resolve some key aspects of flatworm mitochondrial genomes, including generally conserved traits, such as start codons, noncoding regions, the full complement of tRNAs, and whether ATP8 is, or is not, encoded by this extranuclear genome. In an effort to address these difficulties, we sought to determine the mitochondrial transcriptomes and genomes of sexual and asexual taxa of freshwater triclads, a group previously poorly represented in flatworm mitogenomic studies. We have discovered evidence for an alternative start codon, an extended cox1 gene, a previously undescribed conserved open reading frame, long noncoding RNAs, and a highly conserved gene order across the large evolutionary distances represented within the triclads. Our findings contribute to the expansion and refinement of mitogenomics to address evolutionary issues in this diverse group of animals.

KEYWORDS:

Platyhelminthes; Schmidtea mediterranea; atp8; lncRNAs; mitochondria

PMID:
26921295
PMCID:
PMC4856072
DOI:
10.1534/g3.116.028175
[Indexed for MEDLINE]
Free PMC Article

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