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Elife. 2016 Feb 27;5:e12039. doi: 10.7554/eLife.12039.

The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis.

Author information

1
Department of Genetics, Harvard Medical School, Boston, United States.
2
Department of Cell Biology, Harvard Medical School, Boston, United States.
3
MRC Clinical Sciences Centre, Imperial College London, London, United Kingdom.
4
Proteomics facility, MRC Clinical Sciences Centre, Imperial College London, London, United Kingdom.
5
Department of Molecular Biology, Massachusetts General Hospital, Boston, United States.

Abstract

Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling pathway in the C. elegans germline to regulate the disassembly of SC proteins. We find that SYP-2, a SC central region component, is a potential target for MPK-1-mediated phosphorylation and that constitutively phosphorylated SYP-2 impairs the disassembly of SC proteins from chromosomal domains referred to as the long arms of the bivalents. Inactivation of MAP kinase at late pachytene is critical for timely disassembly of the SC proteins from the long arms, and is dependent on the crossover (CO) promoting factors ZHP-3/RNF212/Zip3 and COSA-1/CNTD1. We propose that the conserved MAP kinase pathway coordinates CO designation with the disassembly of SC proteins to ensure accurate chromosome segregation.

KEYWORDS:

<i>c. elegans</i>; MAP kinase; SYP-2; cell biology; chromosomes; crossover recombination; genes; germline; meiosis; synaptonemal complex

PMID:
26920220
PMCID:
PMC4805554
DOI:
10.7554/eLife.12039
[Indexed for MEDLINE]
Free PMC Article

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