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J Cell Sci. 2017 Jan 1;130(1):143-151. doi: 10.1242/jcs.185116. Epub 2016 Feb 26.

Cell polarity defines three distinct domains in pancreatic β-cells.

Author information

1
School of Biomedical Sciences, University of Queensland, St Lucia, Queensland 4072, Australia.
2
Charles Perkins Centre, John Hopkins Drive, University of Sydney, Camperdown, New South Wales, 2050, Australia.
3
Centre for Microscopy and Microanalysis, University of Queensland, St Lucia, Queensland 4072, Australia.
4
Department of Physiology, School of Medical Sciences, The University of Sydney, Camperdown, New South Wales, 2006, Australia.
5
Institute for Molecular Bioscience, University of Queensland, St Lucia, Queensland 4072, Australia.
6
Department of Medicine, University of Toronto, Toronto, Ontario, M5S1A8, Canada.
7
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia.
8
School of Biomedical Sciences, University of Queensland, St Lucia, Queensland 4072, Australia p.thorn@sydney.edu.au.

Abstract

The structural organisation of pancreatic β-cells in the islets of Langerhans is relatively unknown. Here, using three-dimensional (3D) two-photon, 3D confocal and 3D block-face serial electron microscopy, we demonstrate a consistent in situ polarisation of β-cells and define three distinct cell surface domains. An apical domain located at the vascular apogee of β-cells, defined by the location of PAR-3 (also known as PARD3) and ZO-1 (also known as TJP1), delineates an extracellular space into which adjacent β-cells project their primary cilia. A separate lateral domain, is enriched in scribble and Dlg, and colocalises with E-cadherin and GLUT2 (also known as SLC2A2). Finally, a distinct basal domain, where the β-cells contact the islet vasculature, is enriched in synaptic scaffold proteins such as liprin. This 3D analysis of β-cells within intact islets, and the definition of distinct domains, provides new insights into understanding β-cell structure and function.

KEYWORDS:

Diabetes; Insulin; Islet; Polarity; β-Cell

PMID:
26919978
PMCID:
PMC5394774
DOI:
10.1242/jcs.185116
[Indexed for MEDLINE]
Free PMC Article

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