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Plant Signal Behav. 2016;11(4):e1149675. doi: 10.1080/15592324.2016.1149675.

Selective regulation of pyrethrin biosynthesis by the specific blend of wound induced volatiles in Tanacetum cinerariifolium.

Author information

1
a Graduate School of Agriculture, Faculty of Agriculture, Kinki University , Nakamachi, Nara , Japan.
2
b Nara Institute of Science and Technology, Takayama , Ikoma, Nara , Japan.
3
c Kazusa DNA Research Institute , Kazusa-kamatari, Kisarazu, Chiba , Japan.
4
d Research Faculty of Agriculture, Division of Applied Bioscience, Hokkaido University , Kita-ku, Sapporo, Hokkaido , Japan.
5
e Division of Pharmacognosy, College of Pharmaceutical Science, Ritsumeikan University , Noji-higashi, Kusatsu, Shiga , Japan.
6
f Graduate School of Life and Environmental Sciences, Osaka Prefecture University , Nakaku, Sakai, Osaka , Japan.

Abstract

Natural pyrethrins are used to control household and agricultural pests, and it is of value to understand biosynthesis in Tanacetum cinerariifolium for enhanced production. We previously found that a blend of four green leaf volatiles (GLVs) and (E)-β-farnesene emitted by T. cinerariifolium seedlings enhanced gene expressions of certain biosynthetic enzymes in unwounded seedlings; however, the extent to which such a regulation facilitates pyrethrin biosynthesis remains unknown. Here we have investigated the effects of the blend of the volatile organic compounds (VOCs) on gene expressions of seven biosynthetic enzymes. VOC treatment resulted in enhanced chrysanthemyl diphosphate synthase (CDS), chrysanthemic acid synthase (CAS), Tanacetum cinerariifolium GDSL lipase (TcGLIP) and acyl-Coenzyme A oxidase 1 (ACX1) gene expressions that reached a peak at a 12 h VOC treatment, whereas the treatment minimally influenced the expressions of other biosynthetic genes. In undifferentiated Tanacetum tissues, such VOC-induced amplification of CDS, CAS, TcGLIP and ACX1 gene expressions were markedly reduced, suggesting that a high-resolution, VOC-mediated communication is an event selective to differentiated plants.

KEYWORDS:

(E)-β-farnesene; Biosynthesis; gene expression analysis; green Leaf volatiles; pyrethrins; tanacetum cinerariifolium; volatile organic compounds

PMID:
26918634
PMCID:
PMC4883863
DOI:
10.1080/15592324.2016.1149675
[Indexed for MEDLINE]
Free PMC Article

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