Format

Send to

Choose Destination
Science. 2016 Feb 26;351(6276):943-9. doi: 10.1126/science.aad5309.

The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation.

Author information

1
Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
2
Centre de Biologie du Développement, Université Fédérale de Toulouse, Paul Sabatier Campus, 118 Route de Narbonne, 31062 Toulouse, France. bernard.de-massy@igh.cnrs.fr henri-marc.bourbon@univ-tlse3.fr.
3
Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France. bernard.de-massy@igh.cnrs.fr henri-marc.bourbon@univ-tlse3.fr.

Abstract

Meiotic recombination is induced by the formation of DNA double-strand breaks (DSBs) catalyzed by SPO11, the ortholog of subunit A of TopoVI DNA topoisomerase (TopoVIA). TopoVI activity requires the interaction between A and B subunits. We identified a conserved family of plant and animal proteins [the TOPOVIB-Like (TOPOVIBL) family] that share strong structural similarity to the TopoVIB subunit of TopoVI DNA topoisomerase. We further characterize the meiotic recombination proteins Rec102 (Saccharomyces cerevisiae), Rec6 (Schizosaccharomyces pombe), and MEI-P22 (Drosophila melanogaster) as homologs to the transducer domain of TopoVIB. We demonstrate that the mouse TOPOVIBL protein interacts and forms a complex with SPO11 and is required for meiotic DSB formation. We conclude that meiotic DSBs are catalyzed by a complex involving SPO11 and TOPOVIBL.

Comment in

PMID:
26917764
DOI:
10.1126/science.aad5309
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center