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Sci Rep. 2016 Feb 26;6:22032. doi: 10.1038/srep22032.

Label-free imaging to study phenotypic behavioural traits of cells in complex co-cultures.

Author information

1
Phasefocus Ltd, Sheffield, UK.
2
Technology Facility, University of York, UK.
3
Department of Biology, University of York, UK.
4
Centre for Immunology and Infection, York, UK.

Abstract

Time-lapse imaging is a fundamental tool for studying cellular behaviours, however studies of primary cells in complex co-culture environments often requires fluorescent labelling and significant light exposure that can perturb their natural function over time. Here, we describe ptychographic phase imaging that permits prolonged label-free time-lapse imaging of microglia in the presence of neurons and astrocytes, which better resembles in vivo microenvironments. We demonstrate the use of ptychography as an assay to study the phenotypic behaviour of microglial cells in primary neuronal co-cultures through the addition of cyclosporine A, a potent immune-modulator.

PMID:
26915695
PMCID:
PMC4768090
DOI:
10.1038/srep22032
[Indexed for MEDLINE]
Free PMC Article

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