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Phytother Res. 2016 Jun;30(6):929-39. doi: 10.1002/ptr.5595. Epub 2016 Feb 25.

Neurotherapeutic Effects of Pueraria mirifica Extract in Early- and Late-Stage Cognitive Impaired Rats.

Author information

1
Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
2
Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
3
Brain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, PJ46150, Malaysia.
4
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo, Japan.

Abstract

We determined the neurotherapeutic effects of Pueraria mirifica extract (PME) and pure puerarin (PU) in comparison with 17β-estradiol (E2 ) in early- and late-stage cognitive impaired rats. Rats were ovariectomized (OVX), kept for 2 and 4 months to induce early- and late-stage cognitive impairment, respectively, and divided into four groups that were treated daily with (i) distilled water, (ii) 100 mg/kg of PME, (iii) 7 mg/kg of PU, and (iv) 80 µg/kg of E2 for 4 months. The estrogen deficiency symptoms of OVX rats were abrogated by treatment with E2 or PME, but not by treatment with PU. The mRNA level of genes associated with amyloid production (App and Bace1) and hyperphosphorylated Tau (Tau4) were upregulated together with the level of impaired cognition in the 2- and 4-month OVX rats. Treatment with E2 reduced the level of cognitive impairment more than that with PME and PU, and 2-month OVX rats were more responsive than 4-month OVX rats. All treatments down-regulated the Bace1 mRNA level in 2-month OVX rats, while PU and PME also decreased the App mRNA level in 2- and 4-month OVX rats, respectively. Only PU suppressed Tau4 expression in 2-month OVX rats. Thus, PME and PU elicit neurotherapeutic effects in different pathways, and earlier treatment is optimal.

KEYWORDS:

Alzheimer's disease; amyloid plaques; estrogen receptor; menopause; neurofibrillary tangles; spatial memory

PMID:
26915634
DOI:
10.1002/ptr.5595
[Indexed for MEDLINE]

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