Format

Send to

Choose Destination
Genes Cells. 2016 Apr;21(4):358-69. doi: 10.1111/gtc.12349. Epub 2016 Feb 24.

In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration.

Author information

1
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, 739-8526, Hiroshima, Japan.
2
Division of Morphogenesis, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Aichi, Japan.
3
Division of Liberal Arts and Sciences, Aichi Gakuin University, 12 Araike, Iwasaki, Nissin, 470-0195, Aichi, Japan.
4
Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), 38 Nishigonaka, Myodaiji, Okazaki, 445-8585, Aichi, Japan.
5
Spectrography and Bioimaging Facility, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Aichi, Japan.
6
Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan.

Abstract

Xenopus laevis tadpoles can completely regenerate their appendages, such as tail and limbs, and therefore provide a unique model to decipher the molecular mechanisms of organ regeneration in vertebrates. Epigenetic modifications are likely to be involved in this remarkable regeneration capacity, but they remain largely unknown. To examine the involvement of histone modification during organ regeneration, we generated transgenic X. laevis ubiquitously expressing a fluorescent modification-specific intracellular antibody (Mintbody) that is able to track histone H3 lysine 9 acetylation (H3K9ac) in vivo through nuclear enhanced green fluorescent protein (EGFP) fluorescence. In embryos ubiquitously expressing H3K9ac-Mintbody, robust fluorescence was observed in the nuclei of somites. Interestingly, H3K9ac-Mintbody signals predominantly accumulated in nuclei of regenerating notochord at 24 h postamputation following activation of reactive oxygen species (ROS). Moreover, apocynin (APO), an inhibitor of ROS production, attenuated H3K9ac-Mintbody signals in regenerating notochord. Our results suggest that ROS production is involved in acetylation of H3K9 in regenerating notochord at the onset of tail regeneration. We also show this transgenic Xenopus to be a useful tool to investigate epigenetic modification, not only in organogenesis but also in organ regeneration.

PMID:
26914410
DOI:
10.1111/gtc.12349
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center