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Science. 2016 Feb 19;351(6275):841-5. doi: 10.1126/science.aad4925.

DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction.

Author information

1
Institute of Biomaterials and Biomedical Engineering, Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada. Center for Disease Biology and Integrative Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
2
Institute of Biomaterials and Biomedical Engineering, Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.
3
Institute of Biomaterials and Biomedical Engineering, Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada. Department of Chemical Engineering, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada. Department of Material Science and Engineering, University of Toronto, 160 College Street, Room 450, University of Toronto, Toronto, ON M5S 3E1, Canada. warren.chan@utoronto.ca.

Abstract

Precise control of biosystems requires development of materials that can dynamically change physicochemical properties. Inspired by the ability of proteins to alter their conformation to mediate function, we explored the use of DNA as molecular keys to assemble and transform colloidal nanoparticle systems. The systems consist of a core nanoparticle surrounded by small satellites, the conformation of which can be transformed in response to DNA via a toe-hold displacement mechanism. The conformational changes can alter the optical properties and biological interactions of the assembled nanosystem. Photoluminescent signal is altered by changes in fluorophore-modified particle distance, whereas cellular targeting efficiency is increased 2.5 times by changing the surface display of targeting ligands. These concepts provide strategies for engineering dynamic nanotechnology systems for navigating complex biological environments.

PMID:
26912892
DOI:
10.1126/science.aad4925
[Indexed for MEDLINE]
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