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Nat Commun. 2016 Feb 25;7:10811. doi: 10.1038/ncomms10811.

BTG2 bridges PABPC1 RNA-binding domains and CAF1 deadenylase to control cell proliferation.

Stupfler B1,2,3,4, Birck C1,2,3,4, Séraphin B1,2,3,4, Mauxion F1,2,3,4.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France.
2
Centre National de la Recherche Scientifique UMR7104, 67404 Illkirch, France.
3
Institut National de la Santé et de la Recherche Médicale U964, 67404 Illkirch, France.
4
Université de Strasbourg, 67404 Illkirch, France.

Abstract

While BTG2 plays an important role in cellular differentiation and cancer, its precise molecular function remains unclear. BTG2 interacts with CAF1 deadenylase through its APRO domain, a defining feature of BTG/Tob factors. Our previous experiments revealed that expression of BTG2 promoted mRNA poly(A) tail shortening through an undefined mechanism. Here we report that the APRO domain of BTG2 interacts directly with the first RRM domain of the poly(A)-binding protein PABPC1. Moreover, PABPC1 RRM and BTG2 APRO domains are sufficient to stimulate CAF1 deadenylase activity in vitro in the absence of other CCR4-NOT complex subunits. Our results unravel thus the mechanism by which BTG2 stimulates mRNA deadenylation, demonstrating its direct role in poly(A) tail length control. Importantly, we also show that the interaction of BTG2 with the first RRM domain of PABPC1 is required for BTG2 to control cell proliferation.

PMID:
26912148
PMCID:
PMC4773420
DOI:
10.1038/ncomms10811
[Indexed for MEDLINE]
Free PMC Article

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