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Proc Biol Sci. 2016 Feb 24;283(1825):20160042. doi: 10.1098/rspb.2016.0042.

Symbiont-mediated RNA interference in insects.

Author information

1
Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK.
2
Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK p.j.dyson@swansea.ac.uk.

Abstract

RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.

KEYWORDS:

Chagas disease; RNA interference; biocide; insect; symbiotic bacteria

PMID:
26911963
PMCID:
PMC4810840
DOI:
10.1098/rspb.2016.0042
[Indexed for MEDLINE]
Free PMC Article

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