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Eur J Immunol. 2016 May;46(5):1300-3. doi: 10.1002/eji.201646297.

High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.

Author information

1
German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
2
Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
3
Charité - Universitätsmedizin Berlin, Medical Department (Gastroenterology, Infectious Diseases, Rheumatology), Campus Benjamin Franklin, Berlin, Germany.

Abstract

Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.

KEYWORDS:

Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis

PMID:
26909672
PMCID:
PMC5084791
DOI:
10.1002/eji.201646297
[Indexed for MEDLINE]
Free PMC Article

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