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Mycologia. 2016 May-Jun;108(3):533-42. doi: 10.3852/15-226. Epub 2016 Feb 23.

Hyphal tip cytoplasmic organization in four zygomycetous fungi.

Author information

1
School of Life Sciences, Arizona State University, Tempe, Arizona 85287.
2
School of Life Sciences, Arizona State University, Tempe, Arizona 85287 robby@asu.edu.

Abstract

We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.

KEYWORDS:

apical vesicle crescent; cryofixation; electron microscopy; hyphal growth; light microscopy; phylogeny; vesicle organization

PMID:
26908648
DOI:
10.3852/15-226
[Indexed for MEDLINE]

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