Send to

Choose Destination
Mycologia. 2016 May-Jun;108(3):533-42. doi: 10.3852/15-226. Epub 2016 Feb 23.

Hyphal tip cytoplasmic organization in four zygomycetous fungi.

Author information

School of Life Sciences, Arizona State University, Tempe, Arizona 85287.
School of Life Sciences, Arizona State University, Tempe, Arizona 85287


We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.


apical vesicle crescent; cryofixation; electron microscopy; hyphal growth; light microscopy; phylogeny; vesicle organization

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center