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Hum Mol Genet. 2016 May 1;25(9):1867-74. doi: 10.1093/hmg/ddw037. Epub 2016 Feb 9.

Genome-wide association analysis of self-reported events in 6135 individuals and 252 827 controls identifies 8 loci associated with thrombosis.

Author information

1
23andMe, Inc., Mountain View, CA, USA.
2
Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland.
3
Pfizer Worldwide R&D, New York, NY, USA, Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
4
Unitat de Genòmica de Malalties Complexes (UGMC), Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau, IIB-Sant Pau, Barcelona, Spain.
5
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians Universität, Munich, Germany.
6
Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Building 208, DK-2800 Kongens Lyngby, Denmark.
7
Sorbonne Universités, UPMC Univ Paris 06, INSERM UMR_S 1166, Team Genomics & Pathophysiology of Cardiovascular Diseases ICAN Institute for Cardiometabolism and Nutrition, Paris, France.
8
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians University, Munich, Germany, Munich Cluster of Systems Neurology (SyNergy), Munich, Germany.
9
Pfizer Worldwide R&D, New York, NY, USA.
10
Cardiothoracic Surgery Unit, Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden.
11
Unitat d'Hemostàsia i Trombosi, Hospital de la Santa Creu i Sant Pau, IIB-Sant Pau, Barcelona, Spain and.
12
Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
13
Department of Neurology, University of Virginia Health System, Charlottesville, VA, USA.
14
Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, maria.sabater.lleal@ki.se.

Abstract

Thrombotic diseases are among the leading causes of morbidity and mortality in the world. To add insights into the genetic regulation of thrombotic disease, we conducted a genome-wide association study (GWAS) of 6135 self-reported blood clots events and 252 827 controls of European ancestry belonging to the 23andMe cohort of research participants. Eight loci exceeded genome-wide significance. Among the genome-wide significant results, our study replicated previously known venous thromboembolism (VTE) loci near the F5, FGA-FGG, F11, F2, PROCR and ABO genes, and the more recently discovered locus near SLC44A2 In addition, our study reports for the first time a genome-wide significant association between rs114209171, located upstream of the F8 structural gene, and thrombosis risk. Analyses of expression profiles and expression quantitative trait loci across different tissues suggested SLC44A2, ILF3 and AP1M2 as the three most plausible candidate genes for the chromosome 19 locus, our only genome-wide significant thrombosis-related locus that does not harbor likely coagulation-related genes. In addition, we present data showing that this locus also acts as a novel risk factor for stroke and coronary artery disease (CAD). In conclusion, our study reveals novel common genetic risk factors for VTE, stroke and CAD and provides evidence that self-reported data on blood clots used in a GWAS yield results that are comparable with those obtained using clinically diagnosed VTE. This observation opens up the potential for larger meta-analyses, which will enable elucidation of the genetics of thrombotic diseases, and serves as an example for the genetic study of other diseases.

PMID:
26908601
PMCID:
PMC4986325
DOI:
10.1093/hmg/ddw037
[Indexed for MEDLINE]
Free PMC Article

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