Format

Send to

Choose Destination
Oncotarget. 2016 Mar 22;7(12):14064-82. doi: 10.18632/oncotarget.7476.

Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin).

Author information

1
Inserm U892, CNRS UMR6299, University of Nantes, 44007 Nantes, France.
2
Nantes University Hospital, 44007 Nantes, France.
3
Recombinant Protein Core Facility of The University of Nantes, 44007 Nantes, France.
4
Department of Life Sciences, Imperial College London, London SW7, UK.
5
CERMAV-UPR 5301, CNRS, Université Grenoble Alpes, 38041 Grenoble, France.

Abstract

Terminal fucosylated motifs of glycoproteins and glycolipid chains are often altered in cancer cells. We investigated the link between fucosylation changes and critical steps in cancer progression: epithelial-to-mesenchymal transition (EMT) and lymph node metastasis.Using mammary cell lines, we demonstrate that during EMT, expression of some fucosylated antigens (e.g.: Lewis Y) is decreased as a result of repression of the fucosyltransferase genes FUT1 and FUT3. Moreover, we identify the fucose-binding bacterial lectin BC2L-C-Nt as a specific probe for the epithelial state.Prolectin (CLEC17A), a human lectin found on lymph node B cells, shares ligand specificities with BC2L-C-Nt. It binds preferentially to epithelial rather than to mesenchymal cells, and microfluidic experiments showed that prolectin behaves as a cell adhesion molecule for epithelial cells. Comparison of paired primary tumors/lymph node metastases revealed an increase of prolectin staining in metastasis and high FUT1 and FUT3 mRNA expression was associated with poor prognosis. Our data suggest that tumor cells invading the lymph nodes and expressing fucosylated motifs associated with the epithelial state could use prolectin as a colonization factor.

KEYWORDS:

epithelial to mesenchymal transition; fucose; lectins; lymph node metastasis

PMID:
26908442
PMCID:
PMC4924698
DOI:
10.18632/oncotarget.7476
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Impact Journals, LLC Icon for PubMed Central
Loading ...
Support Center