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Virol Sin. 2016 Feb;31(1):49-56. doi: 10.1007/s12250-015-3690-4. Epub 2016 Feb 19.

Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses.

Wang J1,2, Deng F1,2, Ye G1,2, Dong W1,2, Zheng A1,2, He Q1,2,3, Peng G4,5,6.

Author information

1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
2
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
3
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, China.
4
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.
5
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.
6
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.

Abstract

The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins (S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected Vero- CCL-81 (monkey kidney), Huh-7 (human liver), and PK-15 (pig kidney) cells efficiently. CCL94 (cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.

KEYWORDS:

Coronavirus; cell entry; pseudotyped virus; receptor binding; spike proteins

PMID:
26908211
DOI:
10.1007/s12250-015-3690-4
[Indexed for MEDLINE]

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