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Curr Opin Microbiol. 2016 Apr;30:133-138. doi: 10.1016/j.mib.2016.02.003. Epub 2016 Feb 22.

Hfq: the flexible RNA matchmaker.

Author information

1
Division of Molecular and Cellular Biology, NICHD, National Institutes of Health, 18 Library Dr MSC 5430, Bethesda, MD 20892-5430, USA.
2
Division of Molecular and Cellular Biology, NICHD, National Institutes of Health, 18 Library Dr MSC 5430, Bethesda, MD 20892-5430, USA. Electronic address: storzg@mail.nih.gov.

Abstract

The RNA chaperone protein Hfq is critical to the function of small, base pairing RNAs in many bacteria. In the past few years, structures and modeling of wild type Hfq and assays of various mutants have documented that the homohexameric Hfq ring can contact RNA at four sites (proximal face, distal face, rim and C-terminal tail) and that different RNAs bind to these sites in various configurations. These studies together with novel in vitro and in vivo experimental approaches are beginning to give mechanistic insights into how Hfq acts to promote small RNA-mRNA pairing and indicate that flexibility is integral to the Hfq role in RNA matchmaking.

PMID:
26907610
PMCID:
PMC4821791
DOI:
10.1016/j.mib.2016.02.003
[Indexed for MEDLINE]
Free PMC Article

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