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Placenta. 2016 Feb;38:84-92. doi: 10.1016/j.placenta.2015.12.016. Epub 2015 Dec 24.

Fetal asphyctic preconditioning in rats results in a preserved placental inflammatory phenotype at birth.

Author information

1
Department of Pediatrics - Division of Neonatology, Maastricht University Medical Center, Maastricht, The Netherlands; Department of Neuropsychology - Division Neuroscience, Maastricht University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The Netherlands; Child Neurology, Maastricht University Medical Center, Maastricht, The Netherlands.
2
Institute of Biomedicine, Faculty of Medicine, Catholic University of Santiago de Guayaquil, Guayaquil, Ecuador; Enrique C. Sotomayor Obstetrics and Gynecology Hospital, Guayaquil, Ecuador.
3
Department of Pediatrics - Division of Neonatology, Maastricht University Medical Center, Maastricht, The Netherlands; Institute of Biomedicine, Faculty of Medicine, Catholic University of Santiago de Guayaquil, Guayaquil, Ecuador.
4
Institute of Biomedicine, Faculty of Medicine, Catholic University of Santiago de Guayaquil, Guayaquil, Ecuador.
5
Department of Pediatrics - Division of Neonatology, Maastricht University Medical Center, Maastricht, The Netherlands; Department of Neuropsychology - Division Neuroscience, Maastricht University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The Netherlands.
6
Department of Neuropsychology - Division Neuroscience, Maastricht University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The Netherlands; Child Neurology, Maastricht University Medical Center, Maastricht, The Netherlands.
7
Department of Pediatrics - Division of Neonatology, Maastricht University Medical Center, Maastricht, The Netherlands; Institute of Biomedicine, Faculty of Medicine, Catholic University of Santiago de Guayaquil, Guayaquil, Ecuador; Department of Neuropsychology - Division Neuroscience, Maastricht University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The Netherlands. Electronic address: danilo.gavilanes@mumc.nl.

Abstract

INTRODUCTION:

Perinatal asphyxia (PA) is a major cause of neonatal mortality and morbidity. Research has shown that in rats fetal asphyxia (FA) can provoke neuroprotection against a subsequent more severe perinatal asphyctic insult. This is called fetal asphyctic preconditioning (PC). Our objective was to investigate alterations in the placental inflammatory phenotype associated with PC.

METHODS:

FA was induced in the rat at embryonic day 17 by reversibly clamping the uterine circulation and PA was induced at birth by submersion of the uterine horns in a saline bath for 19 min. The effect of PC was studied by inducing FA at E17, followed by PA at E21. Placental TNF-α, IL-1β, IL-6 and IL-10 mRNA and protein levels were measured by qPCR and ELISA.

RESULTS:

IL-1β mRNA increased in the labouring FA group, but IL-1β protein decreased after both FA and PA. In the PC group, IL-1β mRNA and protein levels were similar to controls. IL-6 protein increased 6 h after FA, however decreased 24 h after FA. IL-6 mRNA was higher in the labouring PA group. IL-10 protein decreased 24 h after FA. At birth, IL-10 mRNA increased in the PA group; however, IL-10 protein decreased in both the PA and the FA group. In the PC group, IL-10 mRNA and protein were similar to control levels.

DISCUSSION:

Depleted protein concentrations of IL-10 and IL-1β after one single asphyctic insult were reversed after fetal asphyctic PC. In addition, PC placentas showed less up-regulation of IL-6 mRNA compared to the PA ones. This modulated placental inflammatory phenotype might contribute to the improved neonatal outcome showed after fetal asphyctic PC.

KEYWORDS:

Asphyxia; Fetal; Inflammation; Placenta; Preconditioning

PMID:
26907386
DOI:
10.1016/j.placenta.2015.12.016
[Indexed for MEDLINE]

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