Format

Send to

Choose Destination
See comment in PubMed Commons below
Int J Mol Sci. 2016 Feb 19;17(2):257. doi: 10.3390/ijms17020257.

Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans.

Author information

  • 1Analytical and Environmental Sciences Division, Faculty of Life Sciences & Medicine, King's College London, London SE1 9NH, UK. julie.essig@kcl.ac.uk.
  • 2Medical Research Council-Public Health England (MRC-PHE) Centre for Environment & Health, King's College London, London SE1 9NH, UK. julie.essig@kcl.ac.uk.
  • 3Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. samwebb@slac.stanford.edu.
  • 4Analytical and Environmental Sciences Division, Faculty of Life Sciences & Medicine, King's College London, London SE1 9NH, UK. stephen.sturzenbaum@kcl.ac.uk.
  • 5Medical Research Council-Public Health England (MRC-PHE) Centre for Environment & Health, King's College London, London SE1 9NH, UK. stephen.sturzenbaum@kcl.ac.uk.

Abstract

Environmental metal pollution is a growing health risk to flora and fauna. It is therefore important to fully elucidate metal detoxification pathways. Phytochelatin synthase (PCS), an enzyme involved in the biosynthesis of phytochelatins (PCs), plays an important role in cadmium detoxification. The PCS and PCs are however not restricted to plants, but are also present in some lower metazoans. The model nematode Caenorhabditis elegans, for example, contains a fully functional phytochelatin synthase and phytochelatin pathway. By means of a transgenic nematode strain expressing a pcs-1 promoter-tagged GFP (pcs-1::GFP) and a pcs-1 specific qPCR assay, further evidence is presented that the expression of the C. elegans phytochelatin synthase gene (pcs-1) is transcriptionally non-responsive to a chronic (48 h) insult of high levels of zinc (500 μM) or acute (3 h) exposures to high levels of cadmium (300 μM). However, the accumulation of cadmium, but not zinc, is dependent on the pcs-1 status of the nematode. Synchrotron based X-ray fluorescence imaging uncovered that the cadmium body burden increased significantly in the pcs-1(tm1748) knockout allele. Taken together, this suggests that whilst the transcription of pcs-1 may not be mediated by an exposure zinc or cadmium, it is nevertheless an integral part of the cadmium detoxification pathway in C. elegans.

KEYWORDS:

C. elegans; X-ray fluorescence microscopy; nematode; phytochelatin synthase

PMID:
26907254
PMCID:
PMC4783986
DOI:
10.3390/ijms17020257
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
    Loading ...
    Support Center