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Mucosal Immunol. 2016 Sep;9(5):1303-16. doi: 10.1038/mi.2015.137. Epub 2016 Feb 24.

Human rhinovirus-induced inflammatory responses are inhibited by phosphatidylserine containing liposomes.

Author information

1
Department of Infection, Immunity and Cardiovascular Disease, School of Medicine, Dentistry and Health, University of Sheffield, Sheffield, UK.
2
School of Life and Health Sciences, Aston University, Birmingham, Birmingham, UK.
3
Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
4
School of Medicine, Institute of Infection and Immunity, Cardiff University, Cardiff, UK.
5
Faculty of Science, Department of Biomedical Sciences, University of Sheffield, Sheffield, UK.
6
Department of Pathology, University of Cambridge, Cambridge, UK.
7
School of Biosciences, Cardiff University, Cardiff, UK.

Abstract

Human rhinovirus (HRV) infections are major contributors to the healthcare burden associated with acute exacerbations of chronic airway disease, such as chronic obstructive pulmonary disease and asthma. Cellular responses to HRV are mediated through pattern recognition receptors that may in part signal from membrane microdomains. We previously found Toll-like receptor signaling is reduced, by targeting membrane microdomains with a specific liposomal phosphatidylserine species, 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-L-serine (SAPS). Here we explored the ability of this approach to target a clinically important pathogen. We determined the biochemical and biophysical properties and stability of SAPS liposomes and studied their ability to modulate rhinovirus-induced inflammation, measured by cytokine production, and rhinovirus replication in both immortalized and normal primary bronchial epithelial cells. SAPS liposomes rapidly partitioned throughout the plasma membrane and internal cellular membranes of epithelial cells. Uptake of liposomes did not cause cell death, but was associated with markedly reduced inflammatory responses to rhinovirus, at the expense of only modest non-significant increases in viral replication, and without impairment of interferon receptor signaling. Thus using liposomes of phosphatidylserine to target membrane microdomains is a feasible mechanism for modulating rhinovirus-induced signaling, and potentially a prototypic new therapy for viral-mediated inflammation.

PMID:
26906404
PMCID:
PMC4883656
DOI:
10.1038/mi.2015.137
[Indexed for MEDLINE]
Free PMC Article

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