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Int J Mol Sci. 2016 Feb 18;17(2):251. doi: 10.3390/ijms17020251.

Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.

Author information

1
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. nbaenas@cebas.csic.es.
2
Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. nbaenas@cebas.csic.es.
3
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. piegholdt@foodsci.uni-kiel.de.
4
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. schloesser@foodsci.uni-kiel.de.
5
Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. dmoreno@cebas.csic.es.
6
Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. cgviguera@cebas.csic.es.
7
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. rimbach@foodsci.uni-kiel.de.
8
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. wagner@molecularnutrition.uni-kiel.de.

Abstract

We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.

KEYWORDS:

Brassicaceae; energy metabolism; radish; spargel; sulforaphene

PMID:
26901196
PMCID:
PMC4783981
DOI:
10.3390/ijms17020251
[Indexed for MEDLINE]
Free PMC Article

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