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PLoS One. 2016 Feb 22;11(2):e0149171. doi: 10.1371/journal.pone.0149171. eCollection 2016.

RNA-Seq Reveals the Angiogenesis Diversity between the Fetal and Adults Bone Mesenchyme Stem Cell.

Zhao X1,2, Han Y3,4, Liang Y3,4, Nie C2, Wang J1,2.

Author information

1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, Guangdong Province, 518083, China.
2
BGI-Shenzhen, Shenzhen, Guangdong Province, 518083, China.
3
Binhai Genomics Institute, BGI-Tianjin, Tianjin, Tianjin, 300308, China.
4
Geneis, Beijing, Beijing, 100094, China.

Abstract

In this research, we used RNA sequencing (RNA-seq) to analyze 23 single cell samples and 2 bulk cells sample from human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. The results from the research demonstrated that there were big differences between two cell lines. Adult bone mesenchyme stem cell lines showed a strong trend on the blood vessel differentiation and cell motion, 48/49 vascular related differential expressed genes showed higher expression in adult bone mesenchyme stem cell lines (Abmsc) than fetal bone mesenchyme stem cell lines (Fbmsc). 96/106 cell motion related genes showed the same tendency. Further analysis showed that genes like ANGPT1, VEGFA, FGF2, PDGFB and PDGFRA showed higher expression in Abmsc. This work showed cell heterogeneity between human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. Also the work may give an indication that Abmsc had a better potency than Fbmsc in the future vascular related application.

PMID:
26901069
PMCID:
PMC4764296
DOI:
10.1371/journal.pone.0149171
[Indexed for MEDLINE]
Free PMC Article

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