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Cell Cycle. 2016;15(6):840-9. doi: 10.1080/15384101.2016.1151582.

Combining p53 stabilizers with metformin induces synergistic apoptosis through regulation of energy metabolism in castration-resistant prostate cancer.

Author information

  • 1a Department of Biochemistry , Purdue University , West Lafayette , IN , USA.
  • 2b Department of Dermatology , University of Wisconsin , Madison , WI , USA.
  • 3c Center for Cancer Research, Purdue University , West Lafayette , IN , USA.

Abstract

Since altered energy metabolism is a hallmark of cancer, many drugs targeting metabolic pathways are in active clinical trials. The tumor suppressor p53 is often inactivated in cancer, either through downregulation of protein or loss-of-function mutations. As such, stabilization of p53 is considered as one promising approach to treat those cancers carrying wild type (WT) p53. Herein, SIRT1 inhibitor Tenovin-1 and polo-like kinase 1 (Plk1) inhibitor BI2536 were used to stabilize p53. We found that both Tennovin-1 and BI2536 increased the anti-neoplastic activity of metformin, an inhibitor of oxidative phosphorylation, in a p53 dependent manner. Since p53 has also been shown to regulate metabolic pathways, we further analyzed glycolysis and oxidative phosphorylation upon drug treatments. We showed that both Tennovin-1 and BI2536 rescued metformin-induced glycolysis and that both Tennovin-1 and BI2536 potentiated metformin-associated inhibition of oxidative phosphorylation. Of significance, castration-resistant prostate cancer (CRPC) C4-2 cells show a much more robust response to the combination treatment than the parental androgen-dependent prostate cancer LNCaP cells, indicating that targeting energy metabolism with metformin plus p53 stabilizers might be a valid approach to treat CRPC carrying WT p53.

KEYWORDS:

BI2536; Plk1; SIRT1; Tenovin-1; glycolysis; metformin; oxidative phosphorylation; p53

PMID:
26900800
PMCID:
PMC4845973
[Available on 2017-02-22]
DOI:
10.1080/15384101.2016.1151582
[PubMed - indexed for MEDLINE]

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