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J Sep Sci. 2016 Apr;39(8):1433-41. doi: 10.1002/jssc.201600018. Epub 2016 Apr 6.

Antioxidant activities and liquid chromatography with electrospray ionization tandem mass spectrometry characterization and quantification of the polyphenolic contents of Rumex nervosus Vahl leaves and stems.

Author information

1
Department of Chemistry, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
2
Department of Internal Medicine, Gyeongnam Regional Cancer Center, Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
3
Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
4
Department of Food and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
5
Department of Information Sciences, Research Institute of Natural Sciences, Gyeongsang National University, Jinju, Republic of Korea.
6
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Kwangjin-gu, Seoul, Republic of Korea.
7
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
8
Biochemistry and Chemistry of Nutrition Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
9
Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.

Abstract

In the present study, four compounds, viz. chlorogenic acid, catechin, orientin, and apigenin-O-acetylglycoside among 18 polyphenol compounds (17 flavonoids and one hydroxycinnamic acid derivative) were characterized for the first time in Rumex nervosus leaves and stems by using liquid chromatography with electrospray ionization tandem mass spectrometry. Method validation in terms of determination coefficient, limits of detection, and quantification were ≥ 0.9979, 0.68-1.61, and 2.27-5.38 mg/L, respectively. Accuracy, expressed as percent recovery for two spiking levels (10 and 50 mg/L), were in the range 78.9-110.6% with the exception of caffeic acid. The relative standard deviations were 1-17%. The total polyphenol content was higher by approximately two times in the leaf (1073 mg/kg fresh sample) than in the stem (519.86 mg/kg fresh sample). The antioxidant effects increased in a dose-dependent manner, and the scavenging activities, investigated by measuring 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity, ferrous ions chelating activity, superoxide anion radical scavenging activity, and ferric reducing antioxidant power activity, were significant (p < 0.05) using low concentrations of the leaf extract. Overall, the present study suggests that different parts of R. nervosus have great potential for producing a range of extracts with potential applications in medicine.

KEYWORDS:

Antioxidant activity; Flavonoids; Polyphenols; Rumex nervosus; Tandem mass spectrometry

PMID:
26899192
DOI:
10.1002/jssc.201600018
[Indexed for MEDLINE]

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