Database (Oxford). 2016 Feb 20;2016. pii: bav120. doi: 10.1093/database/bav120. Print 2016.

The UniProtKB guide to the human proteome.

Breuza L¹, Poux S², Estreicher A², Famiglietti ML², Magrane M³, Tognolli M², Bridge A², Baratin D², Redaschi N²; UniProt Consortium.

Author information

1: SIB Swiss Institute of Bioinformatics, Centre Medical Universitaire, 1 Rue Michel Servet, Geneva 4, 1211, Switzerland lionel.breuza@isb-sib.ch.
2: SIB Swiss Institute of Bioinformatics, Centre Medical Universitaire, 1 Rue Michel Servet, Geneva 4, 1211, Switzerland.
3: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK.

Abstract

Advances in high-throughput and advanced technologies allow researchers to routinely perform whole genome and proteome analysis. For this purpose, they need high-quality resources providing comprehensive gene and protein sets for their organisms of interest. Using the example of the human proteome, we will describe the content of a complete proteome in the UniProt Knowledgebase (UniProtKB). We will show how manual expert curation of UniProtKB/Swiss-Prot is complemented by expert-driven automatic annotation to build a comprehensive, high-quality and traceable resource. We will also illustrate how the complexity of the human proteome is captured and structured in UniProtKB. Database URL: www.uniprot.org.