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Methods Mol Biol. 2016;1400:377-85. doi: 10.1007/978-1-4939-3372-3_23.

Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.

Author information

1
Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, Canada, V5Z 1L3. rrebollo@bccrc.ca.
2
Department of Medical Genetics, University of British Columbia, Vancouver, Canada, V6H 3N1. rrebollo@bccrc.ca.
3
Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, Canada, V5Z 1L3. dmager@bccrc.ca.
4
Department of Medical Genetics, University of British Columbia, Vancouver, Canada, V6H 3N1. dmager@bccrc.ca.

Abstract

Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.

KEYWORDS:

DNA methylation; Endogenous retroviruses; In vitro methylation; MeDIP

PMID:
26895065
DOI:
10.1007/978-1-4939-3372-3_23
[Indexed for MEDLINE]

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