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Biotechnol Lett. 2016 May;38(5):761-6. doi: 10.1007/s10529-016-2056-9. Epub 2016 Feb 18.

Determination of glutathione in apoptotic SMMC-7221 cells induced by xylitol selenite using capillary electrophoresis.

Author information

1
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, China Central Normal University, Wuhan, 430079, People's Republic of China.
2
Shijiazhuang Maternal and Child Health Ultrasonography Department, Shijiazhuang, 050011, People's Republic of China.
3
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, China Central Normal University, Wuhan, 430079, People's Republic of China. qichao@mail.ccnu.edu.cn.

Abstract

OBJECTIVE:

To determine the glutathione (GSH) content in a human hepatoma cell line (SMMC-7221) treated with xylitol/selenite, providing a part of an investigation of its anti-cancer mechanisms.

RESULTS:

The nuclei of SMMC-7221 cells were stained with Hoechst 33258 in an apoptosis assay, and their morphology subsequently changed from circular to crescent shape. The calibration curve (r(2) = 0.992) was established, and GSH content markedly decreased after treated with 0.5 and 1 mg xylitol/selenite l(-1) for 12, 36 and 60 h (12 h: from 95.57 ± 19.57 to 29.09 ± 7.74 and 24.27 ± 11.15; 36 h: from 70.73 ± 11.35 to 19.54 ± 6.39 and 9.35 ± 6.69; 60 h: from 72.63 ± 16.94 to 7.432 ± 3.84 and 0). The depletion rate of GSH was more related to the concentration of xylitol/selenite than the treatment time (from 69.95 ± 1.87 to 100 % vs. 0.22 ± 0.2 to 100 %).

CONCLUSIONS:

Xylitol/selenite is a promising anti-cancer drug to induce apoptosis in SMMC-7221 cells. It may regulate the apoptosis through the co-action of multiple mechanisms related to GSH depletion.

KEYWORDS:

Anti-cancer; Apoptosis; Capillary electrophoresis; Glutathione; Laser-induce fluorescence; Selenium; Xylitol/selenite

PMID:
26892224
DOI:
10.1007/s10529-016-2056-9
[Indexed for MEDLINE]

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