Send to

Choose Destination
See comment in PubMed Commons below
Epigenetics. 2016 Mar 3;11(3):227-36. doi: 10.1080/15592294.2016.1146853. Epub 2016 Feb 18.

Epigenome-wide profiling of DNA methylation in paired samples of adipose tissue and blood.

Author information

a Departments of Epidemiology and Biostatistics , Brown University , Providence , RI , USA.
b Department of Epidemiology , Brown University , Providence , RI , USA.
c Department of Epidemiology , Brown University , Providence , RI , USA.
d Department of Molecular Biology , Cell Biology and Biochemistry, Brown University , Providence , RI , USA.
e Departments of Family Medicine and Epidemiology , Brown University , Providence , RI , USA.
f Department of Epidemiology , Brown University , Providence , RI , USA.
g Departments of Epidemiology and Pathobiology , Brown University , Providence , RI , USA.


Many epigenetic association studies have attempted to identify DNA methylation markers in blood that are able to mirror those in target tissues. Although some have suggested potential utility of surrogate epigenetic markers in blood, few studies have collected data to directly compare DNA methylation across tissues from the same individuals. Here, epigenomic data were collected from adipose tissue and blood in 143 subjects using Illumina HumanMethylation450 BeadChip array. The top axis of epigenome-wide variation differentiates adipose tissue from blood, which is confirmed internally using cross-validation and externally with independent data from the two tissues. We identified 1,285 discordant genes and 1,961 concordant genes between blood and adipose tissue. RNA expression data of the two classes of genes show consistent patterns with those observed in DNA methylation. The discordant genes are enriched in biological functions related to immune response, leukocyte activation or differentiation, and blood coagulation. We distinguish the CpG-specific correlation from the within-subject correlation and emphasize that the magnitude of within-subject correlation does not guarantee the utility of surrogate epigenetic markers. The study reinforces the critical role of DNA methylation in regulating gene expression and cellular phenotypes across tissues, and highlights the caveats of using methylation markers in blood to mirror the corresponding profile in the target tissue.


Adipose tissue; DNA methylation; FASN (fatty acid synthase); HIF3A (hypoxia inducible factor 3; blood; body mass index; epigenomics; principal component analysis; surrogate markers

[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for PubMed Central
    Loading ...
    Support Center