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PLoS Biol. 2016 Feb 18;14(2):e1002383. doi: 10.1371/journal.pbio.1002383. eCollection 2016 Feb.

Membrane Potential Dynamics of Spontaneous and Visually Evoked Gamma Activity in V1 of Awake Mice.

Author information

1
Swammerdam Institute for Life Sciences, Center for Neuroscience, Faculty of Science, University of Amsterdam, the Netherlands.
2
Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut, United States of America.
3
Research Priority Program Brain and Cognition, University of Amsterdam, Amsterdam, the Netherlands.
4
Team Waking, Lyon Neuroscience Research Center, INSERM U1028 - CNRS UMR5292 F-69008, Lyon, France.
5
University Lyon 1, F-69000, Lyon, France.

Abstract

Cortical gamma activity (30-80 Hz) is believed to play important functions in neural computation and arises from the interplay of parvalbumin-expressing interneurons (PV) and pyramidal cells (PYRs). However, the subthreshold dynamics underlying its emergence in the cortex of awake animals remain unclear. Here, we characterized the intracellular dynamics of PVs and PYRs during spontaneous and visually evoked gamma activity in layers 2/3 of V1 of awake mice using targeted patch-clamp recordings and synchronous local field potentials (LFPs). Strong gamma activity patterned in short bouts (one to three cycles), occurred when PVs and PYRs were depolarizing and entrained their membrane potential dynamics regardless of the presence of visual stimulation. PV firing phase locked unconditionally to gamma activity. However, PYRs only phase locked to visually evoked gamma bouts. Taken together, our results indicate that gamma activity corresponds to short pulses of correlated background synaptic activity synchronizing the output of cortical neurons depending on external sensory drive.

PMID:
26890123
PMCID:
PMC4758619
DOI:
10.1371/journal.pbio.1002383
[Indexed for MEDLINE]
Free PMC Article

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