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Cell. 1989 Dec 22;59(6):1115-25.

The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene.

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1
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Abstract

The v-myb oncogene induces myeloid leukemias in chickens, transforms myeloid cells in vitro, and encodes a sequence-specific DNA binding protein. We used differential hybridization to screen for v-myb-regulated genes in cells transformed by a temperature-sensitive mutant of the oncogene and identified a new gene, mim-1, which encodes a specifically expressed, secretable protein contained in the granules of both normal and v-myb-transformed promyelocytes. The promoter of the mim-1 gene contains three closely spaced binding sites for v-myb protein and is strongly activated by v-myb in a cotransfection assay. Synthetic copies of the binding sites are both necessary and sufficient to confer v-myb protein-dependent activation to a heterologous promoter. We conclude that mim-1 is a cellular gene that is directly regulated by the product of the v-myb oncogene.

PMID:
2688896
[Indexed for MEDLINE]
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