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Drug Chem Toxicol. 2016 Oct;39(4):445-50. doi: 10.3109/01480545.2016.1141423. Epub 2016 Feb 18.

l-carnitine protects rat hepatocytes from oxidative stress induced by T-2 toxin.

Author information

1
a Department of Pharmacology and Toxicology , School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran .
2
b Department of Toxicology , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran , and.
3
c Department of Food and Drug Analysis , School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.

Abstract

CONTEXT:

Oxidative stress and mitochondrial dysfunction are thought to be the main mechanism of T-2 toxin toxicity. T-2 toxin is the most potent trichothecene mycotoxin which is present in agricultural products. L-carnitine, besides its anti-oxidative properties, facilitates the transportation of long-chain fatty acids in to mitochondrial matrix.

OBJECTIVE:

In this study we tested whether L-carnitine, an antioxidant and a facilitator for long-chain fatty acid transportation across mitochondrial membranes, could protect rat hepatocytes against toxicity induced by T-2 toxin.

MATERIALS AND METHODS:

L-carnitine in low and high doses (50 and 500 mg/kg) was administered for five consecutive days to male Wistar rats. Hepatocytes were isolated and freshly exposed to appropriate concentration of T-2 toxin for 2 h followed by oxidative stress and cell death evaluations.

RESULTS:

Glutathione depletion, ROS overproduction and mitochondrial membrane potential collapse were determined under T-2 toxin exposure. Pretreatment with L-carnitine particularly at high-dose reduced toxicity and prevented the hepatocytes from abnormal caspase-3 activity and apoptosis.

CONCLUSION:

Low toxicity of L-carnitine and its mitochondrial protective effects promises an effective way to reduce or prevent the toxicity induced by certain environmental pollutants, including T-2 toxin.

KEYWORDS:

T-2 toxin; apoptosis; l-carnitine; mitochondria; rat hepatocyte

PMID:
26888052
DOI:
10.3109/01480545.2016.1141423
[Indexed for MEDLINE]

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