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J Endocrinol Invest. 2016 Jul;39(7):785-91. doi: 10.1007/s40618-016-0435-7. Epub 2016 Feb 17.

Aberrant expression and hormonal regulation of Galectin-3 in endometriosis women with infertility.

Author information

1
Department of Reproductive Endocrinology, Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, Shanghai, 200011, China.
2
Department of Gynecology, Chongqing Ninth People's Hospital, Chongqing, China.
3
Department of Chemical and Biomedical Engineering, University of South Florida, Tampa, FL, 33620, USA.
4
Department of Medicine, SUNY Downstate Medical Center, New York, NY, USA.
5
Department of Reproductive Endocrinology, Obstetrics and Gynecology Hospital, Fudan University, 413 Zhaozhou Road, Shanghai, 200011, China. zhang.wei.fudan@gmail.com.
6
Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, NH, USA. Chao.Cheng@dartmouth.edu.

Abstract

OBJECTIVE:

To investigate the role and potential molecular mechanism of Galectin-3 (Gal-3) in the etiology of endometriosis-associated infertility.

METHODS:

We detected Gal-3 expression in eutopic endometrium from women with endometriosis-associated infertility and healthy women without endometriosis or infertility. We then evaluated Gal-3 expression in endometrial glandular epithelial cells (EECs) and endometrial stromal cells (ESCs) and investigated its response to hormone stimulation in EECs and ESCs from both groups of women.

RESULTS:

Results of real-time PCR and western blot analysis showed Gal-3 expression in both proliferative and secretory stages of the menstrual cycle decreased significantly in women with endometriosis-associated infertility compared to healthy women. The changes in expression of Gal-3 were more dramatic in EECs than ESCs. Moreover, estrogen (E2) and progesterone (P4) induced Gal-3 expression in EECs of healthy groups, and P4 was more significant than E2 and combined E2 and P4 (E2P4). However, in the endometriosis group, P4 failed to induce a similar increase in Gal-3 expression.

CONCLUSIONS:

Our results suggest that aberrant expression of Gal-3 might contribute to infertility in patients with endometriosis due to progesterone resistance.

KEYWORDS:

Endometriosis; Galectin-3 (Gal-3); Sex hormone; Uterine receptivity

PMID:
26886939
PMCID:
PMC4906070
DOI:
10.1007/s40618-016-0435-7
[Indexed for MEDLINE]
Free PMC Article

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