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Mol Cell Biochem. 2016 Mar;414(1-2):115-27. doi: 10.1007/s11010-016-2665-6. Epub 2016 Feb 17.

Response of the JAK-STAT pathway to mammalian hibernation in 13-lined ground squirrel striated muscle.

Author information

1
Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada.
2
Department of Surgery & Center for Engineering in Medicine, Massachusetts General Hospital & Harvard Medical School, Building 114 16th Street, Charlestown, MA, 02129, USA.
3
New Beginnings Ob/Gyn, 193 Mountain Avenue, Springfield, NJ, 07081, USA.
4
Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada. kenneth_storey@carleton.ca.

Abstract

Over the course of the torpor-arousal cycle, hibernators must make behavioral, physiological, and molecular rearrangements in order to keep a very low metabolic rate and retain organ viability. 13-lined ground squirrels (Ictidomys tridecemlineatus) remain immobile during hibernation, and although the mechanisms of skeletal muscle survival are largely unknown, studies have shown minimal muscle loss in hibernating organisms. Additionally, the ground squirrel heart undergoes cold-stress, reversible cardiac hypertrophy, and ischemia-reperfusion without experiencing fatal impairment. This study examines the role of the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway in the regulation of cell stress in cardiac and skeletal muscles, comparing euthermic and hibernating ground squirrels. Immunoblots showed a fivefold decrease in JAK3 expression during torpor in skeletal muscle, along with increases in STAT3 and 5 phosphorylation and suppressors of cytokine signaling-1 (SOCS1) protein levels. Immunoblots also showed coordinated increases in STAT1, 3 and 5 phosphorylation and STAT1 inhibitor protein expression in cardiac muscle during torpor. PCR analysis revealed that the activation of these pro-survival signaling cascades did not result in coordinate changes in downstream genes such as anti-apoptotic B-cell lymphoma-2 (Bcl-2) family gene expression. Overall, these results indicate activation of the JAK-STAT pathway in both cardiac and skeletal muscles, suggesting a response to cellular stress during hibernation.

KEYWORDS:

Anti-apoptosis; Heart; Ictidomys tridecemlineatus; JAK-STAT signal transduction; Mammalian hibernation; Muscle

Comment in

PMID:
26885984
DOI:
10.1007/s11010-016-2665-6
[Indexed for MEDLINE]

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