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Ann Biomed Eng. 2016 Jun;44(6):1970-82. doi: 10.1007/s10439-016-1566-x. Epub 2016 Feb 16.

Design of pH-Responsive Biomaterials to Enable the Oral Route of Hematological Factor IX.

Horava SD1,2, Peppas NA3,4,5,6,7.

Author information

1
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
2
Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, Austin, TX, USA.
3
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.
4
Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.
5
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.
6
Department of Surgery and Perioperative Care, Dell Medical School, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.
7
Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.

Abstract

The oral administration of hematological factor IX (FIX) can offer a convenient prophylactic treatment for hemophilia B patients. pH-Responsive hydrogels based on poly(methacrylic acid)-grafted-poly(ethylene glycol) (P(MAA-g-EG)) have been engineered as delivery vehicles for FIX. In oral delivery, such hydrogel carriers protected FIX from the gastric environment and released it under intestinal conditions as demonstrated by evaluation of the loading and release of FIX. Tailoring of the hydrogel networks improved the loading of FIX within the microcarriers, which is critical for minimizing protein degradation. Optimizing the loading conditions by increasing the incubation time and using a reduced ionic strength buffer further improved the delivery potential of the microcarriers. The presence of the microcarriers significantly enhanced the oral absorption of FIX in vitro. As shown in this work, P(MAA-g-EG) microcarriers are promising candidates for the oral delivery of FIX.

KEYWORDS:

Biomaterials; Drug delivery; Factor IX; Hydrogels

PMID:
26883955
PMCID:
PMC4880530
DOI:
10.1007/s10439-016-1566-x
[Indexed for MEDLINE]
Free PMC Article

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