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ACS Appl Mater Interfaces. 2016 Feb;8(7):4378-84. doi: 10.1021/acsami.5b10792. Epub 2016 Feb 15.

Multiple-Armed Tetrahedral DNA Nanostructures for Tumor-Targeting, Dual-Modality in Vivo Imaging.

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Division of Physical Biology & Bioimaging Center, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
Department of Nuclear Medicine, Zhongshan Hospital, Fudan University , Shanghai 200032, China.
School of Chemistry and Biology Engineering, Changsha University of Science and Technology , Changsha 410004, China.
UCB Pharma , Slough, SL1 14EN Berkshire, United Kingdom.


In this work, we have developed multiple-armed DNA tetrahedral nanostructures (TDNs) for dual-modality in vivo imaging using near-infrared (NIR) fluorescence and single-photon emission computed tomography (SPECT). We found that the presence of arm strands in TDNs remarkably enhanced their in vitro stability, allowing them to stay intact for at least 12 h in serum. By using NIR fluorescence imaging, we evaluated in mice the pharmacokinetics of TDNs, which exhibited distinctly different in vivo biodistribution patterns compared with those of double-stranded (ds)DNA. We also noticed that TDNs had twofold longer circulation time in the blood system than that of dsDNA. With the use of multiple-armed TDNs, we could precisely anchor an exact number of functional groups including tumor-targeting folic acid (FA), NIR emitter Dylight 755, and radioactive isotope (99m)Tc on prescribed positions of TDNs, which showed the capability of targeted imaging ability in cancer cells. Furthermore, we realized noninvasive tumor-targeting imaging in tumor-bearing mice by using both NIR and SPECT modalities.


dual-modality imaging; near-infrared fluorescence; single-photon emission computed tomography; tetrahedral DNA nanostructures; tumor targeting

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