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Nat Methods. 2016 Apr;13(4):325-8. doi: 10.1038/nmeth.3770. Epub 2016 Feb 15.

Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.

Author information

1
Neurosciences Program, Stanford University, Stanford, California, USA.
2
Department of Electrical Engineering, Stanford University, Stanford, California, USA.
3
Department of Bioengineering, Stanford University, Stanford, California, USA.
4
Department of Neurochemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
5
Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan.
6
Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
7
Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California, USA.

Abstract

Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.

PMID:
26878381
PMCID:
PMC5717315
DOI:
10.1038/nmeth.3770
[Indexed for MEDLINE]
Free PMC Article

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