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Res Cardiovasc Med. 2015 Dec 23;5(1):e29134. doi: 10.5812/cardiovascmed.29134. eCollection 2016 Feb.

Polymorphisms in NOS3, MTHFR, APOB and TNF-α Genes and Risk of Coronary Atherosclerotic Lesions in Iranian Patients.

Author information

1
Department of Biology, Faculty of Science, Yazd University, Yazd, IR Iran.
2
Department of Cardiac Surgery, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran.
3
Department of Biology, Ashkezar Branch, Islamic Azad University, Ashkezar, IR Iran.

Abstract

BACKGROUND:

Atherosclerosis is a complex multifocal arterial disease involving interactions between multiple genetic and environmental factors.

OBJECTIVES:

In the present study, we investigated the possible association between NOS3 (rs1799983), MTHFR (rs1801133), APOB (rs5742904) and TNF-α (rs361525) polymorphisms and the risk of coronary atherosclerotic lesions in Iranian patients.

PATIENTS AND METHODS:

In the case-control study, 108 patients with coronary atherosclerosis disease and 95 control subjects with no family history of cardiovascular disease were enrolled. Genotypes for NOS3, MTHFR, APOB and TNF-α polymorphisms were identified using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).

RESULTS:

We specifically detected the NOS3 TT genotype in 12 patients (11.11%) and did not find the same genotype in any of the controls. The frequencies of T allele in patients and the controls were 24% and 17.8%, respectively. The prevalence of the MTHFR TT genotype was 16.7% in patients and 2.2% in control groups. The prevalence of the APOB-100 (R3500Q) mutation in this patient population was 0%. The frequency of the A allele in the TNF-α gene was 11.1% and 11% in patients and controls, respectively, and the AA genotype was undetected.

CONCLUSIONS:

Our results show a significant association of NOS3 and MTHFR gene polymorphisms with coronary atherosclerotic lesions. Therefore, these variants might influence the risk of coronary artery disease, specifically in the Iranian population.

KEYWORDS:

APOB; Atherosclerosis; MTHFR; NOS3; PCR-RFLP; Polymorphism; TNF-α

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