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Endocrine. 2016 Aug;53(2):423-32. doi: 10.1007/s12020-016-0886-9. Epub 2016 Feb 13.

Differential modulation of cytosolic lipases activities in liver and adipose tissue by high-carbohydrate diets.

Author information

1
Department of Nutrition, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil.
2
Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
3
Department of Morphology, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
4
Laboratory of Physiology, Federal University of São João del-Rei, Avenida Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, Minas Gerais, 35501-296, Brazil. valeria.chaves@gmail.com.

Abstract

Several studies have demonstrated that a high-fructose (FRUC) diet induces metabolic and haemodynamic abnormalities, known as the metabolic syndrome, which are characterised by obesity, glucose intolerance, insulin resistance, dyslipidaemia and hypertension. In this study, the effect of a FRUC diet (60 % fructose) for 8 weeks on the metabolism of lipids in liver and epididymal adipose tissue from Wistar rats was compared with the AIN-93M diet and the effects of the AIN-93M diet were compared with a chow diet. The FRUC diet induced marked increases in both hepatocyte lipid droplet volume and postprandial serum levels of triacylglycerol (TAG), but reduced the postprandial serum levels of insulin. The AIN-93M diet induced marked increases in the hepatocyte lipid droplet volume and the serum levels of insulin, without affecting the serum levels of TAG. We found that isocaloric substitution of cornstarch, dextrinised cornstarch and sucrose (AIN-93M diet) for fructose did not affect the hepatic VLDL-TAG secretion and adipose tissue glucose uptake, lipolysis and cytosolic lipases activities in rats. However, the high-fructose diet induced a severe steatosis in liver accompanied by a decrease in cytosolic lipases activities. In adipose tissue, the FRUC diet induced a decrease in the lipoprotein lipase activity, and an increase in lipogenesis. FRUC and AIN-93M diets induced changes in lipid homeostasis in liver and adipose tissue by distinct biochemical mechanisms.

KEYWORDS:

Adipose tissue; Cytosolic lipases activities; High-fructose diet; Non-alcoholic fatty liver

PMID:
26874528
DOI:
10.1007/s12020-016-0886-9
[Indexed for MEDLINE]

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