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J Exp Bot. 2016 Apr;67(8):2127-38. doi: 10.1093/jxb/erw015. Epub 2016 Feb 11.

Natural variation in rosette size under salt stress conditions corresponds to developmental differences between Arabidopsis accessions and allelic variation in the LRR-KISS gene.

Author information

1
Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Postbus 94215, 1090GE Amsterdam, the Netherlands.
2
Department of Phytopathology, University of Amsterdam, Postbus 94215, 1090GE Amsterdam, the Netherlands.
3
Biosystematics Group, Wageningen University and Research Centre, Wageningen, the Netherlands.
4
Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Postbus 94215, 1090GE Amsterdam, the Netherlands c.s.testerink@uva.nl.

Abstract

Natural variation among Arabidopsis accessions is an important genetic resource to identify mechanisms underlying plant development and stress tolerance. To evaluate the natural variation in salinity stress tolerance, two large-scale experiments were performed on two populations consisting of 160 Arabidopsis accessions each. Multiple traits, including projected rosette area, and fresh and dry weight were collected as an estimate for salinity tolerance. Our results reveal a correlation between rosette size under salt stress conditions and developmental differences between the accessions grown in control conditions, suggesting that in general larger plants were more salt tolerant. This correlation was less pronounced when plants were grown under severe salt stress conditions. Subsequent genome wide association study (GWAS) revealed associations with novel candidate genes for salinity tolerance such as LRR-KISS (At4g08850),flowering locus KH-domain containing protein and a DUF1639-containing protein Accessions with high LRR-KISS expression developed larger rosettes under salt stress conditions. Further characterization of allelic variation in candidate genes identified in this study will provide more insight into mechanisms of salt stress tolerance due to enhanced shoot growth.

KEYWORDS:

Arabidopsis; GWAS; development; natural variation; rosette size; salt stress.

PMID:
26873976
PMCID:
PMC4809279
DOI:
10.1093/jxb/erw015
[Indexed for MEDLINE]
Free PMC Article

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