Format

Send to

Choose Destination
Cytoskeleton (Hoboken). 2016 Mar;73(3):109-16. doi: 10.1002/cm.21284. Epub 2016 Mar 7.

Rapid centriole assembly in Naegleria reveals conserved roles for both de novo and mentored assembly.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, California.
2
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California.
3
Department of Biology, Brandeis University, Waltham, Massachusetts.

Abstract

Centrioles are eukaryotic organelles whose number and position are critical for cilia formation and mitosis. Many cell types assemble new centrioles next to existing ones ("templated" or mentored assembly). Under certain conditions, centrioles also form without pre-existing centrioles (de novo). The synchronous differentiation of Naegleria amoebae to flagellates represents a unique opportunity to study centriole assembly, as nearly 100% of the population transitions from having no centrioles to having two within minutes. Here, we find that Naegleria forms its first centriole de novo, immediately followed by mentored assembly of the second. We also find both de novo and mentored assembly distributed among all major eukaryote lineages. We therefore propose that both modes are ancestral and have been conserved because they serve complementary roles, with de novo assembly as the default when no pre-existing centriole is available, and mentored assembly allowing precise regulation of number, timing, and location of centriole assembly.

KEYWORDS:

Naegleria; basal bodies; centriole biogenesis; flagellum development

PMID:
26873879
DOI:
10.1002/cm.21284
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center