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J Ethnopharmacol. 2016 Apr 2;181:221-8. doi: 10.1016/j.jep.2016.01.040. Epub 2016 Feb 9.

Tagetes lucida Cav.: Ethnobotany, phytochemistry and pharmacology of its tranquilizing properties.

Author information

1
Laboratorio de Neurofarmacología de Productos Naturales. Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatría Ramón de Fuente, Calz. México-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370 México, D.F., México; Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Del. Coyoacán, C.P. 04510 México D.F., México.
2
Laboratorio de Neurofarmacología de Productos Naturales. Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatría Ramón de Fuente, Calz. México-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370 México, D.F., México. Electronic address: evag@imp.edu.mx.
3
Laboratorio de Neurofarmacología de Productos Naturales. Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatría Ramón de Fuente, Calz. México-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370 México, D.F., México.
4
Laboratorio de Neurofarmacología de Productos Naturales. Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatría Ramón de Fuente, Calz. México-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370 México, D.F., México; Laboratorio Multidisciplinario de Investigación. Escuela Militar de Graduados de Sanidad. SEDENA, México, D.F. México.
5
Postgrado en Botánica, Colegio de Postgraduados, Carr. México-Texcoco Km. 36.5, Montecillo, 56230 Estado de México, México.
6
Instituto de Química, Universidad Nacional Autónoma de México, Del. Coyoacán, C.P. 04510 México D.F., México.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Morelos State is one of the most important regions of Mexico where several plant species are used in traditional medicine to influence central nervous system (CNS) activity; for example Tagetes lucida Cav.

AIM OF THE STUDY:

To investigate the ethnobotany, phytochemistry and pharmacology of the tranquilizing properties of T. lucida aerial parts.

MATERIAL AND METHODS:

Data on the medicinal uses of T. lucida were explored by interviewing healers and merchants of local markets in different regions of Morelos State by using a questionnaire. Anxiolytic and/or sedative-like responses of the T. lucida were investigated in experimental models in mice such as: open-field, exploration cylinder, hole-board, plus-maze, and the barbituric-induced hypnosis potentiation. The possible mechanism of action was explored in the presence of WAY100635 (0.32mg/kg, i.p.) and flumazenil (10mg/kg, i.p.) antagonists. A feasible active compound was isolated and identified by using conventional chromatography, including UHPLC and MS (DART) [M+H]+ techniques.

RESULTS:

Interviews of healers and merchants from ten local regions of Morelos State showed that they recommended T. lucida as infusion and as tincture for several culture-bound syndromes associated with the CNS. Anxiolytic and sedative-like activities of polar extracts were corroborated in the experimental models; these effects were inhibited in the presence of 5-HT1A and GABA/BDZ receptor antagonists. Dimethylfraxetin was identified as one possible active compound.

CONCLUSIONS:

The results support the anxiolytic and sedative-like properties of T. lucida in traditional medicine by involving serotonergic and GABAergic neurotransmission and coumarinic constituents.

KEYWORDS:

Anxiety; Central nervous system; Coumarins; Diazepam (PubChem CID: 3016); Dimethylfraxetin (6,7,8-Trimethoxycoumarin) (PubChem CID: 3083928); Ethnobotany; Flumazenil (PubChem CID: 3373); Sedative; Sodium pentobarbital (PubChem CID: 23676152); Tagetes lucida; WAY100635 maleate salt (PubChem CID: 11957721)

PMID:
26873624
DOI:
10.1016/j.jep.2016.01.040
[Indexed for MEDLINE]

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