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Plant Cell Physiol. 2016 Apr;57(4):848-61. doi: 10.1093/pcp/pcw030. Epub 2016 Feb 12.

Haspin has Multiple Functions in the Plant Cell Division Regulatory Network.

Author information

1
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
2
College of Bioscience and Biotechnology, Chubu University, Matsumoto-cho, Kasugai, Aichi, 478-8501 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
3
Division of Biological Science, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan JST, CREST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
4
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
5
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan kuri@bio.nagoya-u.ac.jp.

Abstract

Progression of cell division is controlled by various mitotic kinases. In animal cells, phosphorylation of histone H3 at Thr3 by the kinase Haspin (haploid germ cell-specific nuclear protein kinase) promotes centromeric Aurora B localization to regulate chromosome segregation. However, less is known about the function of Haspin in regulatory networks in plant cells. Here, we show that inhibition of Haspin with 5-iodotubercidin (5-ITu) in Bright Yellow-2 (BY-2) cells delayed chromosome alignment. Haspin inhibition also prevented the centromeric localization of Aurora3 kinase (AUR3) and disrupted its function. This suggested that Haspin plays a role in the specific positioning of AUR3 on chromosomes in plant cells, a function conserved in animals. The results also indicated that Haspin and AUR3 are involved in the same pathway, which regulates chromosome alignment during prometaphase/metaphase. Remarkably, Haspin inhibition by 5-ITu also led to a severe cytokinesis defect, resulting in binuclear cells with a partially formed cell plate. The 5-ITu treatment did not affect microtubules, AUR1/2 or the NACK-PQR pathway; however, it did alter the distribution of actin filaments on the cell plate. Together, these results suggested that Haspin has several functions in regulating cell division in plant cells: in the localization of AUR3 on centromeres and in regulating late cell plate expansion during cytokinesis.

KEYWORDS:

Aurora3 kinase; BY-2; Chromosome alignment; Cytokinesis; Haspin

PMID:
26872832
DOI:
10.1093/pcp/pcw030
[Indexed for MEDLINE]

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