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J Biophotonics. 2017 Feb;10(2):271-277. doi: 10.1002/jbio.201500267. Epub 2016 Feb 12.

Kinetic analysis of nanoparticle-protein interactions using a plasmon waveguide resonance.

Author information

1
Department of Electrical and Computer Engineering, University of Toronto, Ontario, M5S 3G4, Canada.
2
Department of Engineering Physics, EcolePolytechnique de Montreal, Montreal, H3C 3A7, Canada.

Abstract

A plasmon waveguide resonance (PWR) sensor is proposed for studying the interaction between gold nanoparticles and proteins. The ability of the PWR sensor to operate in both TM and TE Polarizations, i.e. its polarization diversity, facilitates the simultaneous spectroscopy of the nanoparticles surface reactions using both polarizations. The response of each polarization to streptavidin-biotin binding at the surface of gold nanoparticles is investigated in real time. Finally, using the principles of multimode spectroscopy, the nanoparticle's surface reactions are decoupled from the bulk solution refractive index variations. Schematic diagram of the NP-modified PWR sensor.

KEYWORDS:

Biosensing; Plasmon waveguide resonance; Protein-nanoparticle interactions; SPR sensor

PMID:
26871886
DOI:
10.1002/jbio.201500267
[Indexed for MEDLINE]

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